The main goal of the research was to investigate and explain what impact the registration of cultural assets from the area of Split-Dalmatia County on the representative lists of World Tangible and Intangible Cultural Heritage had on the development of tourism in Split-Dalmatia County. The aim of the empirical research was to determine the views of the key stakeholders of the tourist offer on the importance of registering cultural assets on the World Heritage list for the development of sustainable cultural tourism, as well as to determine the views on the acceptance and implementation of guidelines for the development of sustainable cultural tourism. The rapid and often insufficiently controlled development of tourism can have negative effects on tourist destinations in the long term. In moments of increasingly frequent uncertainty and unexpected changes at the global level, sustainable development should be a logical and primary solution for many tourist destinations in order to successfully fight the challenges that arise every day. In destinations with a rich and valuable cultural heritage, such as the Split-Dalmatia County, the uncontrolled development of tourism destroys valuable monumental heritage and makes it impossible for future generations to use and enjoy it. That is why the development of sustainable cultural tourism should represent a priority direction of development. The results of the research showed that there is no difference in the views of different stakeholders of the tourist offer on the importance of registering cultural assets on the World Heritage list for the development of sustainable cultural tourism, and that the Split-Dalmatia County should strive for the development of sustainable cultural tourism. The research showed that the guidelines for the development of sustainable cultural tourism are most accepted by state, regional, or local administration bodies, and least by civil society organizations, although it is important to emphasize that the limiting fact of this result is the number of respondents in certain stakeholder groups. Considering that sustainable development implies balance and harmony between all sectors, and puts the local community in the foreground, it is necessary to increase the education of all stakeholders about the importance of developing sustainable cultural tourism. Greater application of the guidelines for the development of sustainable cultural tourism would fulfill the opinion of the respondents that the Split-Dalmatia County should strive for the development of sustainable cultural tourism and would accelerate its development. Only by the joint action of all the stakeholders of the tourist offer in cooperation with the local community, sustainable development of cultural tourism, which is desirable and necessary for the Split-Dalmatia County, can be effectively influenced.