Non-profit organizations are becoming the topic of scientific papers more often and the reason for this is their significant increase in the recent years. What significantly distinguishes non-profit organizations from those in the for-profit sector is that the purpose of their existence is not to make a profit but to increase the social well-being. Non-profit organizations are determined by their mission, which is the purpose of their existence. One of the specifics of these organizations are multiple stakeholders, multiple stakeholders are users, donors, volunteers, board of directors, public and employees. Multiple stakeholders are actually the target groups to which non-profit organizations should direct their marketing activities, but it is important to emphasize that a separate marketing strategy is needed for each group. The nonprofit sector has experienced significant growth and organizations must compete for scarce resources. In order to stand out from the competition, nonprofits need to use marketing tools in their business even though some feel that the use of marketing goes against their mission because the funds are not used to fulfill the mission. It is important to emphasize that the proper use of marketing tools results in additional resources because the funds used for marketing will be used to attract new resources. One of the key stakeholders of nonprofits are certainly volunteers. Without volunteers and their invested time and effort, non-profit organizations would not be able to fulfill their mission and survive in the market. There is a growing interest in attracting volunteers, and in order to achieve this, non-profit organizations need to know what motivates their volunteers. Volunteer motivation can be divided into two types of motives, altruistic and egoistic. Altruistic motives are those that motivate volunteers to take action out of a desire to benefit others, while egoistic motives are those that motivate volunteers to take action out of a desire to increase their own well-being. Nonprofits can use these types of motivations to attract volunteers but also keep the existing ones. One way to motivate volunteers by applying marketing tools is using the marketing mix. For this reason, this paper examines the impact of the marketing mix on volunteer motivation. The results showed that volunteers who think that the marketing mix of the non-profit organization in which they volunteered is good quality have a greater presence of both altruistic and egoistic motivations than those who think that the marketing mix of the non-profit organization in which they volunteered is poor or underdeveloped. This confirms the importance of using and shaping the marketing mix of non-profit organizations.